3D Cell Culture Day Kruibeke

Culturing cells outside of their natural environment in a laboratory under controlled conditions is essential to scientific research with the 2D cell culture method being established over a century ago. However, 2D cell culture, although extremely valuable, has its limitations in answering more complex biological questions. This led to the development of the 3D cell culture methodology which often provides more accurate and physiologically relevant tissue models. During this seminar we will be presenting:

• Benefits of 3D versus traditional cell culture technologies.
• Different 3D cell culture techniques.
• 3D cell culture, nomenclature and different structures.

When it comes to mimicking human disease, traditional 2D cell culture and animal models are often inadequate and limited in their physiological relevance. Cells grown in 3D more closely mimic in vivo behavior in tissues and organs. In recent years, three-dimensional (3D) cell culture models have emerged as the preferred in vitro cancer model as they better mimic the in vivo tumor microenvironment. For instance, 3D cultures, specifically spheroids, develop hypoxic cores and demonstrate gradients of soluble factors and a diffusion profile for drugs like solid tumors. During this seminar we will discuss the following topics:

• Spheroid culture and techniques to obtain these structures.
• Recommendations and optimization during 3D spheroid culture.
• Application of Corning products in 3D spheroid culture to answer biological questions.

Countless medical breakthroughs over the past century have begun with two-dimensional (2D) cell culture models. However, the limitations of 2D culture are obvious as cells, tissues and organs exist in three dimensions, and tissues almost always consist of more than one cell type with their own spatial organization and function. By providing greater physiological relevance, 3D cultures narrow the bridge between in vitro and live-animal or human assays. In this scenario, organoids show promise for their physiologic-like complexity, as they capture the spatial organization of heterogeneous tissue-specific cells, cell-cell interactions, cell-matrix interactions, and certain in vivo physiological functions generated by tissue-specific cells (which contain adult stem cells). During this seminar we will discuss:

• Organoid Biology, how to obtain organoids and their relevance.
• Key Corning products for organoid culture.
• Major optimization steps of organoid culture.
• Application of Corning products in Organoid culture to answer biological complex questions.

Discover how 3D cell culture is reshaping modern research. This workshop provides an accessible overview of 3D models, focusing on spheroid and organoid culture techniques. Through expert presentations and a hands-on session, you’ll learn practical workflows, key optimization steps, and how Corning solutions support reliable and physiologically relevant 3D results. Ideal for researchers looking to strengthen or expand their 3D culture expertise with practical experience.